You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1252204 |
---|---|
Category | Antibodies |
Description | ENO2 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | ENO2/1375 |
Tested applications | FC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG2b |
Immunogen | Amino acids 416-433 of human Neuron Specific Enolase were used as the immunogen for this NSE antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Western blot: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlIF: 1-2 ug/mlIHC (FFPE): 0.1-0.2 ug/ml for 30 min at RTThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NSE antibody to be titered up or down for optimal performance. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | ENO2 |
UniProt ID | P09104 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | ENO2, Enolase 2, Enolase 2 (gamma, neuronal), Neur Read more... |
Note | For research use only |
Application notes | Western blot: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlIF: 1-2 ug/mlIHC (FFPE): 0.1-0.2 ug/ml for 30 min at RTThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NSE antibody to be titered up or down for optimal performance. |
Expiration Date | 12 months from date of receipt. |
IHC testing of FFPE human cerebellum with NSE antibody (clone ENO2/1375). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.
IHC testing of FFPE human pheochromocytoma with NSE antibody (clone ENO2/1375). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.
Western blot testing of human 1) Y79, 2) HeLa and 3) HepG2 cell lysate with NSE antibody (clone ENO2/1375). Predicted molecular weight ~47 kDa.
IHC testing of FFPE mouse pancreas with NSE antibody (clone ENO2/1375). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.
IHC testing of FFPE rat heart with NSE antibody (clone ENO2/1375). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.
SDS-PAGE Analysis of Purified, BSA-Free NSE Antibody (clone ENO2/1375). Confirmation of Integrity and Purity of the Antibody.
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating