NSE Antibody / Neuron Specific Enolase

• Catalog Number: orb386134
• Category: Antibodies
• Description: Recognizes a protein of about 50kDa, which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: ENO2/1375
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG2b, kappa
• Immunogen: Amino acids 416-433 of human Neuron Specific Enolase were used as the immunogen for this NSE antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.2-0.4ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This NSE antibody is available for research use only.
• Entrez: 2026
• UniProt ID: P09104
• Storage: Store the NSE antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NSE antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human cerebellum using NSE antibody

Immunohistochemical staining of Human pheochromocytoma using NSE antibody

Western blot analysis of human Y79(lane 1), HeLa(lane 2) and HepG2(lane 3) cell lysate using NSE antibody

Immunohistochemical staining of Mouse pancreas using NSE antibody

Immunohistochemical staining of Rat heart using NSE antibody