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Catalog Number | orb1252289 |
---|---|
Category | Antibodies |
Description | FOXA1 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | FOXA1/1514 |
Tested applications | FC, IF, IHC, WB |
Reactivity | Human |
Isotype | IgG2b, kappa |
Immunogen | A human partial recombinant protein corresponding to amino acids 372-472 was used as the immunogen for this FOXA1 antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Flow Cytometry: 0.5-1ug/10^6 cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 1-2 ug/ml for 30 min at RTThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the FOXA1 antibody to be titered up or down for optimal performance. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | FOXA1 |
UniProt ID | P55317 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | FOXA1 Antibody: HNF3A, TCF3A, HNF3A, Hepatocyte nu Read more... |
Note | For research use only |
Application notes | Flow Cytometry: 0.5-1ug/10^6 cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 1-2 ug/ml for 30 min at RTThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the FOXA1 antibody to be titered up or down for optimal performance. |
Expiration Date | 12 months from date of receipt. |
IHC testing of FFPE human prostate carcinoma with FOXA1 antibody (clone FOXA1/1514). HIER: boil sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.
IHC testing of FFPE human prostate carcinoma with FOXA1 antibody (clone FOXA1/1514). HIER: boil sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.
SDS-PAGE Analysis of Purified, BSA-Free FOXA1 Antibody (clone FOXA1/1514). Confirmation of Integrity and Purity of the Antibody.
ELISA, FC, IF, WB | |
Bovine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
ELISA, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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