FOXA1 Antibody

• Catalog Number: orb1251970
• Category: Antibodies
• Description: FOXA1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: FHBA1-1
• Tested applications: FC, IF, IHC
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: A human partial recombinant protein corresponding to amino acids 372-472 was used as the immunogen for this FOXA1 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1ug/10^6 cellsIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 min at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the FOXA1 antibody to be titered up or down for optimal performance.1. Staining of FFPE tissue is enhanced by boiling sections in 10mM Tris with 1mM EDTA, pH9 for 10-20 min followed by cooling at RT for 20 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: FOXA1
• UniProt ID: P55317
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: FOXA1 Antibody: HNF3A, TCF3A, HNF3A, Hepatocyte nu
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1ug/10^6 cellsIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 min at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the FOXA1 antibody to be titered up or down for optimal performance.1. Staining of FFPE tissue is enhanced by boiling sections in 10mM Tris with 1mM EDTA, pH9 for 10-20 min followed by cooling at RT for 20 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human bladder carcinoma with FOXA1 antibody.

IHC testing of FFPE human prostate carcinoma with FOXA1 antibody.

IHC testing of FFPE rat lung with FOXA1 antibody.

IHC testing of FFPE rat colon with FOXA1 antibody.