FOXA1 antibody

• Catalog Number: orb18549
• Category: Antibodies
• Description: Goat polyclonal antibody to FOXA1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, WB
• Reactivity: Bovine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 10ug/ml, FACS: 10ug/ml
• Conjugation: Unconjugated
• MW: 49.1
• Target: FOXA1 / HNF3A
• Entrez: 3169
• Protein Sequence: GVYSRPVLNTS
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti FOXA1 antibody, anti HNF3A antibody, anti for
• Note: For research use only
• Application notes: WB: Approx 50kDa band observed in nuclear lysates of cell line HepG2 and approx 50-55kDa in Mouse Liver and in nuclear lysates of cell line MCF7 calculated MW of 49.1kDa according to Human NP_004487.2 and 48.9kDa according to Mouse NP_032285.2).These bands we. Notes: Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 and U2OS cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10u
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human Prostate using FOXA1 antibody

Immunohistochemical staining of Human Breast using FOXA1 antibody

Western blot analysis of HepG2 (A) and (0.3ug/ml) MCF7 (B) nuclear cell lysate using FOXA1 antibody.

Western blot analysis of Mouse Liver lysate using FOXA1 antibody.

Immunofluorescence analysis of MCF7 cells using FOXA1 antibody.

Immunofluorescence analysis of U2OS cells using FOXA1 antibody.

Flow cytometric analysis of MCF7 cells using FOXA1 antibody.