FOXA1 Antibody

• Catalog Number: orb1252290
• Category: Antibodies
• Description: FOXA1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: FOXA1/1512
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: A human partial recombinant protein corresponding to amino acids 372-472 was used as the immunogen for this FOXA1 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1ug/10^6 cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 1-2 ug/ml for 30 min at RTOptimal dilution of the FOXA1 antibody should be determined by the researcher.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: FOXA1
• UniProt ID: P55317
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: FOXA1 Antibody: HNF3A, TCF3A, HNF3A, Hepatocyte nu
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1ug/10^6 cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 1-2 ug/ml for 30 min at RTOptimal dilution of the FOXA1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human prostate carcinoma with FOXA1 antibody (clone FOXA1/1512). HIER: boil sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.

IHC testing of FFPE human prostate with FOXA1 antibody (clone FOXA1/1512). HIER: boil sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.

Western blot testing of human 1) MCF7 (breast) and 2) PC3 (prostate) cell lysate with FOXA1 antibody (clone FOXA1/1512). Predicted molecular weight: ~49 kDa.

SDS-PAGE Analysis of Purified, BSA-Free FOXA1 Antibody (clone FOXA1/1512). Confirmation of Integrity and Purity of the Antibody.