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Catalog Number | orb750424 |
---|---|
Category | Antibodies |
Description | Fluorescein antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Isotype | Antiserum |
Immunogen | Fluorescein conjugated Bovine Serum Albumin (BSA) |
Concentration | 90 mg/mL |
Dilution range | ELISA: 1:10,000 - 1:50,000, IHC: User Optimized, WB: 1:1,000 - 1:5,000 |
Form/Appearance | Lyophilized |
Purity | Fluorescein Antibody was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, Fluorescein conjugated goat IgG and Fluorescein conjugated KLH. |
Conjugation | Unconjugated |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | goat anti-Fluorescein Antibody, goat anti-FITC ant Read more... |
Note | For research use only |
Application notes | Anti-Fluorescein Antibody has been assayed against 1.0 ug of Fluorescein conjugated Rabbit IgG in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Donkey) code #605-703-125 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:100,000 to 1:500,000 of the reconstitution concentration is suggested for this product. |
Expiration Date | 12 months from date of receipt. |
DOT, ELISA, FC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
FITC |
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