Beta galactosidase antibody (FITC)

• Catalog Number: orb344713
• Category: Antibodies
• Description: Beta galactosidase antibody (FITC)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, IF, WB
• Isotype: IgG
• Immunogen: Beta Galactosidase (E. coli)
• Concentration: 1.0 mg/mL
• Dilution range: IF: User Optimized, WB: User Optimized
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Beta Galactosidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum and purified and partially purified Beta Galactosidase (E. coli).
• Conjugation: FITC
• UniProt ID: P00722
• NCBI: NP_414878.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Beta Galactosidase Antibody fluorescei
• Note: For research use only
• Application notes: Anti-Beta Galactosidase Fluorescein Conjugated Antibody has been tested by dot bot and western blot and is designed for fluorescent western blotting and immunofluorescence. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead) using Beta Galactosidase antibody (FITC)

Western blot analysis of shows 80 ng and lane 2 shows 20 ng loaded onto gel using Beta Galactosidase antibody (FITC)

Western blot analysis of shows detection of a band at ~117 kDa (lane 1) corresponding to b-Gal present in a partially purified preparation (arrowhead) using Beta Galactosidase antibody (FITC)