AKT3 FITC antibody (FITC)

• Catalog Number: orb344535
• Category: Antibodies
• Description: AKT3 FITC antibody (FITC)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 25F6.F6.D8
• Tested applications: DOT, ELISA, FC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1
• Immunogen: Anti-AKT3 Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography using a synthetic peptide corresponding to internal residues of human AKT3 protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: User Optimized, FC: User Optimized, IHC: User Optimized, IF: User Optimized, WB: User Optimized
• Form/Appearance: Lyophilized
• Purity: Anti-AKT3 antibody is directed against human AKT3. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT3 antibody was purified from tissue culture by Protein A chromatography. Cross reactivity with AKT3 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT2 and AKT has not been determined.
• Conjugation: FITC
• UniProt ID: Q9Y243
• NCBI: NP_001193658.1
• Storage: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Mouse anti-AKT3 antibody FITC conjugation, fluores
• Note: For research use only
• Application notes: Anti-AKT3 FITC Antibody has been tested by ELISA and dot blot and is suitable for Flow Cytometry, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT3 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of mouse liver tissue using AKT3 FITC antibody (FITC)

Western blot analysis of GST Tagged recombinant using AKT3 FITC antibody (FITC)

Dot Blot analysis of 100 ng (Lane1), 33.3 ng (Lane2), 11.1 ng (Lane3), 3.70 ng (Lane4), 1.23 ng (Lane5) using AKT3 FITC antibody (FITC)