FGFR2 Antibody

• Catalog Number: orb1263054
• Category: Antibodies
• Description: FGFR2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: This FGFR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-51 amino acids from the N-terminal region of human FGFR2.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 92 kDa
• Target: FGFR2
• UniProt ID: P21802
• NCBI: P21802
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Fibroblast growth factor receptor 2, FGFR-2, K-sam
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000-1:2000For IF starting dilution is: 1:25For IHC-P starting dilution is: 1:25For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:1000-1:2000 dilution Lane 1: DU145 whole cell lysate Lane 2: A549 whole cell lysate Lane 3: T47D whole cell lysate Lane 4: Hela whole cell lysate Lane 5: K562 whole cell lysate Lane 6: M.brain whole lysate Lysates/proteins at 20 ug per lane.

Fluorescent image of Hela cells stained with FGFR2 Antibody (N-term). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Immunohistochemical analysis of paraffin-embedded H. liver section using FGFR2 Antibody (N-term). Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. brain section using FGFR2 Antibody (N-term). Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Western blot analysis in mouse NIH-3T3 cell line lysates (35 ug/lane).

Confocal immunofluorescent analysis of FGFR2 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.