FBXW2 antibody

• Catalog Number: orb18575
• Category: Antibodies
• Description: Goat polyclonal antibody to FBXW2
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:128000, WB: 0.3-1.5 μg/ml, IHC-P: 2-3µg/ml
• Conjugation: Unconjugated
• MW: 51.5
• Target: FBXW2
• Entrez: 26190
• Protein Sequence: KRGSSFLAGEHPG
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti FBXW2 antibody, anti F-box and WD-40 domain p
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Kidney, shows cytoplasmic staining of epithelial cells in some renal tubules. Recommended concentration, 2-3ug/ml.WB: Approx 48kDa band observed in Human Kidney lysates (calculated MW of 48.0kDa according to NP_036296.1). Recommended concentration: 0.3-1.5 μg/ml. A stronger band of unknown identity was also consistently observed at appr. 30kDa - both bands were successfully blocked by incubation with the immunising peptide.
• Expiration Date: 12 months from date of receipt.

orb18575 staining (0.3ug/ml) of Human Kidney lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb18575 (3ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.