EPHA2 Antibody

• Catalog Number: orb1263100
• Category: Antibodies
• Description: EPHA2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Monkey
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This EphA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-60 amino acids from the N-terminal region of human EphA2.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50For IHC-P starting dilution is: 1:50~100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 108 kDa
• Target: EPHA2
• UniProt ID: P29317
• NCBI: P29317
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Ephrin type-A receptor 2, Epithelial cell kinase,
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50For IHC-P starting dilution is: 1:50~100
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of hEPHA2-T45 in MCF-7 cell line lysates (35 ug/lane)

Western blot analysis of hEPHA2-T45 in mouse NIH-3T3 cell line lysates (35 ug/lane)

Western blot analysis of EPHA2 using rabbit polyclonal hEPHA2-T45 using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA2 gene (Lane 2)

Flow cytometric analysis of NCI-H292 cells using EphA2 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.