EGR1 Antibody

• Catalog Number: orb1258074
• Category: Antibodies
• Description: EGR1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant protein of human EGR1
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 57kDa
• Target: EGR1
• UniProt ID: P18146
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: EGR1, AT225, G0S30, KROX-24, NGFI-A, TIS8, ZIF-268
• Note: For research use only
• Application notes: WB: 1:200 - 1:1000
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using EGR1 antibody (orb1258074) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.