EEA1 Antibody

• Catalog Number: orb402272
• Category: Antibodies
• Description: EEA1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence of human EEA1 (RENQSLQIKHTQALNRKWAEDNEVQN).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunocytochemistry/Immunofluorescence, 5μg/ml Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 170 kDa
• UniProt ID: Q15075
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Early endosome antigen 1; Endosome-associated prot
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A431 cells using anti-EEA1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB:Lane 1:human HeLa cell; 2:human placenta tissue; 3:human Colo320 cell; 4:human 22RV1 cell; 5:human A549 cell; 6:rat brain tissue; 7:rat lung tissue; 8:mouse spleen tissue; 9:mouse brain tissue; 10:mouse HEPA1-6 cell; 11:mouse NIH/3T3 cell.

IF analysis of EEA1 using anti-EEA1 antibody. EEA1 was detected in immunocytochemical section of A431 cells.