EEA1 Antibody

• Catalog Number: orb1527593
• Category: Antibodies
• Description: EEA1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: between 100 and 150
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:200 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: EEA1
• UniProt ID: Q15075
• NCBI: NP_003557.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: early endosome antigen 1; early endosome antigen 1
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human EEA1 by western blot. Samples: Whole cell lysate from HeLa (5, 15, and 50 µg) and HEK293T (T; 50 µg) cells. Antibody: Affinity purified rabbit anti-EEA1 antibody orb1527593 used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human EEA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EEA1 antibody orb1527593 used for IP at 3 µg/mg lysate.

Detection of human EEA1 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-EEA1 (Cat. No. orb1527593) used at a dilution of 1: 200 (1µg/ml). Detection: DAB