EEA1 Antibody

• Catalog Number: orb1294269
• Category: Antibodies
• Description: Rabbit polyclonal antibody to EEA1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rabbit
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the C-terminus region.
• Dilution range: Western Blot 1:2000-1:4000 Immunofluorescence 1:500-1:1000
• Conjugation: Unconjugated
• Target: EEA1
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-EEA1 antibody - at 1/2000 dilution, Lysates at 35 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294269 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human testis, breast, Colorental and lymph node cancer tissue labeling EEA1 with orb1294269 at 1/100.

EEA1 antibody by immunofluorescent analysis. Sample: MCF-7 cell. Red: EEA1 antibody (orb1294269) diluted at 1:500. Blue: DAPI.

EEA1 antibody by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons. Green: EEA1 antibody (orb1294269) diluted at 1:500. Red: Tuj1 antibody. Blue: DAPI.