EEA1 antibody

• Catalog Number: orb195341
• Category: Antibodies
• Description: Goat polyclonal antibody to EEA1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Human
• Dilution range: ELISA: 1:128000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml
• Conjugation: Unconjugated
• MW: 162
• Target: EEA1 (aa821-835)
• Entrez: 8411
• Protein Sequence: QETKIQHEELNNRIQ
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti EEA1 antibody, anti early endosome antigen 1
• Note: For research use only
• Application notes: WB: Approx 200kDa band observed in lysates of cell line HEK293 (calculated MW of 162kDa according to NP_003557.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1µg/ml. Primary incubatiion 1 hour at room temperat. Notes: Immunofluorescence :Strong expression of the protein seen in the vesicles/cytoplasm of NIH3T3 cells. Recommended concentration: 5µg/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HEK293 lysate using EEA1 antibody