EEA1 Antibody

• Catalog Number: orb1527589
• Category: Antibodies
• Description: EEA1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse
• Immunogen: between 1361 and 1411
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - Not recommended. Use rabbit anti-EEA1 antibody A301-896A or A301-897A.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: EEA1
• UniProt ID: Q15075
• NCBI: NP_003557.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: early endosome antigen 1; early endosome antigen 1
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse EEA1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) , and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-EEA1 antibody orb1527589 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. EEA1 was immunoprecipitated by rabbit anti-EEA1 antibodies, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).