E4F1 Antibody

• Catalog Number: orb2636935
• Category: Antibodies
• Description: E4F1 (E4F transcription factor 1), also known as E4F, is a 784 amino acid protein that localizes to both the nucleus and the cytoplasm and contains 9 C2H2-type zinc fingers. Expressed ubiquitously in adult and fetal tissues, E4F1 exists as a homodimer that binds DNA and is thought to act as a transcriptional repressor and may also play a role in cell survival and growth via cell cycle control.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-E4F1-2D1
• Tested applications: FACS, IF
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Recombinant full-length human E4F1 protein was used as the immunogen for the E4F1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This E4F1 antibody is available for research use only.
• UniProt ID: Q66K89
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the E4F1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of PFA-fixed MCF7 cells using E4F1 antibody (green, clone PCRP-E4F1-2D1) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with E4F1 antibody (blue, clone PCRP-E4F1-2D1), and unstained cells (red).

SDS-PAGE analysis of purified, BSA-free E4F1 antibody (clone PCRP-E4F1-2D1) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using E4F1 antibody (clone PCRP-E4F1-2D1). These results demonstrate the foremost specificity of the PCRP-E4F1-2D1 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.