E4F1 Antibody

• Catalog Number: orb1151652
• Category: Antibodies
• Description: Mouse monoclonal antibody to E4F1
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-E4F1-2D1
• Tested applications: FACS, IF
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Recombinant full-length human E4F1 protein was used as the immunogen for the E4F1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This E4F1 antibody is available for research use only.
• UniProt ID: Q66K89
• Storage: Aliquot the E4F1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the E4F1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of PFA-fixed MCF7 cells using E4F1 antibody (green, clone PCRP-E4F1-2D1) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with E4F1 antibody (blue, clone PCRP-E4F1-2D1), and unstained cells (red).

SDS-PAGE analysis of purified, BSA-free E4F1 antibody (clone PCRP-E4F1-2D1) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using E4F1 antibody (clone PCRP-E4F1-2D1). These results demonstrate the foremost specificity of the PCRP-E4F1-2D1 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.