E4F1 monoclonal antibody (M03), clone 4B10

• Catalog Number: orb2293397
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a partial recombinant E4F1.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4B10
• Tested applications: ELISA, IF, WB
• Reactivity: Human
• Isotype: IgG2a Kappa
• Immunogen: E4F1 (NP_004415.2, 685 a.a. ~ 784 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: GHQIIVQNVTMDEETALGPEAAAADTITIATPESLTEQVAMTLASAISEGTVLAARAGTSGTEQATVTMVSSEDIEILEHAGELVIASPEGQLEVQTVIV
• NCBI: NP_004415.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged E4F1 is 0.1 ng/ml as a capture antibody.

Immunofluorescence of monoclonal antibody to E4F1 on HeLa cell. [antibody concentration 10 ug/ml]

Western Blot analysis of E4F1 expression in transfected 293T cell line by E4F1 monoclonal antibody (M03), clone 4B10. Lane 1: E4F1 transfected lysate (83.5 KDa). Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (36.74 KDa).