Cytokeratin 14 antibody

Catalog Number: orb98255

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CategoryPrimary antibodies
Catalog Numberorb98255
Species/HostMouse
ClonalityMonoclonal
Tested applicationsIHC-Fr, WB
ReactivityCanine, Human, Porcine, Rat
Clone IDRCK107
IsotypeIgG1
Form/AppearanceEach vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
ConjugationUnconjugated
UniProt IDP02533
Application notesRCK107 is suitable for immunocytochemistry, immunohistochemistry on frozen sections, flow cytometry and immunoblotting. Optimal antibody dilution should be determined by titRation; recommended range is 1:100-1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100-1:1000 for immunoblotting appliCations.
StorageThe antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
DescriptionMouse monoclonal to Cytokeratin 14
Expiration Date12 months from date of receipt.
NoteFor research use only.
SourceRCK107 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a Mouse immunized with a cytoskeletal preparation of TR146 epithelial cells.
  1. 6 images
    • Immunohistochemical staining of rat brain tissue using pan Cytokeratin antibody.
    • Immunohistochemical staining of mouse lung tissue using pan Cytokeratin antibody.
    • Immunohistochemical staining of mouse lung tissue using pan Cytokeratin antibody.
    • Immunohistochemical staining of Mouse brain tissue using pan Cytokeratin antibody.
    • Immunohistochemical staining of Mouse brain tissue using pan Cytokeratin antibody.
    • Immunohistochemical staining of human colon cancer tissue using pan Cytokeratin antibody.
    pan Cytokeratin antibody [orb158093] Custom conjugation service available

    ELISA, ICC, IF, IHC

    Bovine, Equine, Human, Mouse, Rat

    100 μl, 200 μl, 50 μl
  2. 5 images
    • Immunofluorescence analysis of Rat skin lysates using KRT14 antibody
    • Immunohistochemical staining of Mouse kidney tissue using KRT14 antibody
    • Immunohistochemical staining of Human esophagus tissue using KRT14 antibody
    • Immunohistochemical staining of Rat lung tissue using KRT14 antibody
    • Western blot analysis of extracts of various cell lines lysates using KRT14 antibody
    KRT14 antibody [orb540173]

    IF, WB, IHC

    Mouse

    Rabbit

    Polyclonal

    50 μl, 100 μl, 200 μl
  3. 4 images
    • Immunohistochemical analysis of paraffin embedded human breast cancer tissue using Cytokeratin 14 antibody
    • IHC-P of human epithelial cells (Cytokeratin 14 antibody at 1:300)
    • Immunohistochemical analysis of formalin fixed and paraffin embedded rat articular cartilage tissue using Cytokeratin 14 antibody
    • IHC-P of rat skin tissue (Cytokeratin 14 antibody at 1:300)
    Cytokeratin 14 antibody [orb13340] Custom conjugation service available

    ELISA, IF, WB, IHC

    Bovine, Canine, Equine, Mouse, Rabbit, Rat, Sheep

    100 μl, 200 μl, 50 μl
  4. 3 images
    • Western blot analysis of MCF7 (Lane 1), mouse brain (Lane 2), rat brain (Lane 3) whole cell lysates using KRT14 antibody
    • Immunohistochemical analysis of formalin-fixed and paraffin-embedded human breast cancer tissue using KRT14 antibody (dilution at 1:200)
    • Immunofluorescense analysis of MCF7 cells lysates using KRT14 antibody
    KRT14 antibody [orb214169] Custom conjugation service available

    ICC, IF, IHC-P, WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    30 μl, 100 μl, 200 μl
  5. 3 images
    • Immunohistochemical analysis of formalin-fixed and paraffin-embedded human prostate (10X) tissue using Cytokeratin 14 antibody
    • Immunohistochemical analysis of formalin-fixed and paraffin-embedded human prostate (20X) tissue using Cytokeratin 14 antibody
    • Western blot analysis of A431 cell lysate using Cytokeratin 14 antibody
    Cytokeratin 14 Antibody / CK14 / KRT14 [orb248446]

    FACS, IF, IHC-P

    Human, Mouse, Rat

    Mouse

    Monoclonal

    Unconjugated

    20 μg, 100 μg