Cytokeratin 18 Antibody

• Catalog Number: orb606908
• Category: Antibodies
• Description: This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, also called KRT18 and CK18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: KRT18/2808R
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Full length recombinant human protein was used as the immunogen for the recombinant CK18 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant CK18 antibody is available for research use only.
• UniProt ID: P05783
• Storage: Store the recombinant CK18 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant CK18 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human colon carinoma with recombinant CK18 antibody (clone KRT18/2808R). Required HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human colon carinoma with recombinant CK18 antibody (clone KRT18/2808R). Required HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human thyroid carinoma with recombinant CK18 antibody (clone KRT18/2808R). Required HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min and allow to cool before testing.

Immunofluorescent staining of permeabilized human MCF7 cells with recombinant CK18 antibody antibody (clone KRT18/2808R, green) and Reddot nuclear stain (red).

Western blot testing of human HCT116 antibody with recombinant CK18 antibody (clone KRT18/2808R). Predicted molecular weight ~48 kDa.

Flow cytometry testing of permeabilized human HeLa cells with CK18 antibody (clone KRT18/2808R); Red = isotype control, Blue = anti-CK18 antibody.

SDS-PAGE analysis of purified, BSA-free recombinant CK18 antibody (clone KRT18/2808R) as confirmation of integrity and purity.