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Catalog Number | orb1260421 |
---|---|
Category | Antibodies |
Description | CYCS Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human Cytochrome c (NP_061820.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 14kDa |
Target | CYCS |
UniProt ID | P99999 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | CYCS, CYC, HCS, THC4, Cytochrome c, Cytochrome c, Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using Cytochrome c antibody (orb1260421) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat liver using Cytochrome c antibody (orb1260421) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using Cytochrome c antibody (orb1260421) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using Cytochrome c antibody (orb1260421) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using Cytochrome c antibody (orb1260421) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using Cytochrome c antibody (orb1260421) at dilution of 1:150 (40x lens).
Immunofluorescence analysis of HeLa cells using Cytochrome c antibody (orb1260421) at dilution of 1:100. Blue: DAPI for nuclear staining.
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, ICC, IF, IHC, IHC-Fr, WB | |
Hamster | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Rat, Zebrafish | |
Rabbit | |
Polyclonal | |
Unconjugated |
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