Cytochrome C CYCS Antibody (monoclonal, 15F10)

• Catalog Number: orb443140
• Category: Antibodies
• Description: Cytochrome C CYCS Antibody (monoclonal, 15F10)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 15F10
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG1
• Immunogen: E.coli-derived human Cytochrome C recombinant protein (Position: G2-E105). Human Cytochrome C shares 91% amino acid (aa) sequence identity with both mouse and rat Cytochrome C.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 14 kDa
• UniProt ID: P99999
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Cytochrome c; CYCS; CYC
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of K562 cells using anti-Cytochrome C antibody (Blue line).Isotype control antibody (Green line) was mouse IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of A431 cells using anti-Cytochrome C antibody (Blue line).Isotype control antibody (Green line) was mouse IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of A431 cells using anti-Cytochrome C antibody (Blue line).Isotype control antibody (Green line) was mouse IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis using anti-Cytochrome C antibody.Lane 1:rat brain Tissue;2:rat heart Tissue;3:rat kidney Tissue;4:rat testis Tissue;5:mouse brain Tissue;6:mouse heart Tissue;7:mouse kidney Tissue;8:mouse testis Tissue;9:mouse Neuro-2a Cell.

WB analysis of Cytochrome C using anti-Cytochrome C antibody.Lane 1:human HeLa Cell;2:human HepG2 cell;3:human K562 Cell;4:human Caco-2 Cell.

IF analysis of Cytochrome C using anti-Cytochrome C antibody. Cytochrome C was detected in immunocytochemical section of MCF7 cells.

IHC analysis of Cytochrome C using anti-Cytochrome C antibody.Cytochrome C was detected in paraffin-embedded section of human intestinal cancer tissue.

IHC analysis of Cytochrome C using anti-Cytochrome C antibody.Cytochrome C was detected in paraffin-embedded section of human mammary cancer tissue.

IHC analysis of Cytochrome C using anti-Cytochrome C antibody.Cytochrome C was detected in paraffin-embedded section of human lung cancer tissue.