Cytochrome C/CYCS Antibody

• Catalog Number: orb610893
• Category: Antibodies
• Description: Cytochrome C/CYCS Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Monkey, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Cytochrome C (91-105 aa ERADLIAYLKKATNE), identical to the related mouse and rat sequences.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat, Monkey Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, Rat, Mouse Immunocytochemistry , 0.5-1μg/ml, Human, - Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry, 1-μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 11749 MW
• UniProt ID: P99999
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Cytochrome c;CYCS;CYC;
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of CACO-2 cells using anti-CYCS antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG.Unlabelled sample (Red line) was also used as a control.

IF analysis of CYCS using anti-CYCS antibody.

IF analysis of CYCS using anti-CYCS antibody.

IHC analysis of Cytochrome C using anti-Cytochrome C antibody.

IHC analysis of Cytochrome C using anti-Cytochrome C antibody.

Anti-Cytochrome C antibody.Lane 1:Rat Liver Tissue Lysate at 50ug, Lane 2:HELA cell Lysate at 40ug, Lane 3:MCF-7 cell Lysate at 40ug, Lane 4:HEPA cell Lysate at 40ug

Anti-Cytochrome C antibody.

Anti-Cytochrome C antibody, IHC(F): Rat Brain Tissue

Anti-Cytochrome C antibody, IHC(P):Rat Lung Tissue