Cytochrome C/CYCS Antibody

• Catalog Number: orb251517
• Category: Antibodies
• Description: Cytochrome C/CYCS Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Cytochrome C recombinant protein (Position: G2-E105). Human Cytochrome C shares 91% amino acid (aa) sequence identity with both mouse and rat Cytochrome C.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry , 0.5-1μg/ml, Human, - Immunofluorescence, 2μg/ml, Human, Mouse, Rat, -
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 11749 MW
• UniProt ID: P99999
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Cytochrome c;CYCS;CYC;
• Note: For research use only
• Application notes: WB: The detection limit for Cytochrome C is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of Cytochrome C using anti-Cytochrome C antibody.Lane 1:Rat Brain Tissue;2:Mouse Brain Tissue;3:Rat Cardiac Muscle Tissue;4:Mouse Cardiac Muscle Tissue;5:U87 Cell;6:NEURO Cell;7:HELA Cell;8:JURKAT Cell;9:Human Placenta Tissue.

IF analysis of Cytochrome C using anti-Cytochrome C antibody.Cytochrome C was detected in paraffin-embedded section of mouse cardiac muscle tissues.

IF analysis of Cytochrome C using anti-Cytochrome C antibody.Cytochrome C was detected in paraffin-embedded section of human intestinal cancer tissues.

IF analysis of Cytochrome C using anti-Cytochrome C antibody.Cytochrome C was detected in paraffin-embedded section of rat cardiac muscle tissues.

IHC analysis of Cytochrome C using anti-Cytochrome C antibody.Cytochrome C was detected in paraffin-embedded section of Mouse Brain Tissue.

IHC analysis of Cytochrome C using anti-Cytochrome C antibody.Cytochrome C was detected in paraffin-embedded section of Rat Brain Tissue.

IHC analysis of Cytochrome C using anti-Cytochrome C antibody.Cytochrome C was detected in paraffin-embedded section of Human Intestinal Cancer Tissue.

IHC analysis of Cytochrome C using anti-Cytochrome C antibody.Cytochrome C was detected in immunocytochemical section of SMMC-7721.