Cyclin D1 antibody

• Catalog Number: orb387896
• Category: Antibodies
• Description: Mouse monoclonal antibody to Cyclin D1
• Clonality: Monoclonal
• Clone Number: DCS-6
• Tested applications: FC, ICC, IHC-Fr, IHC-P, IP, WB
• Reactivity: Human, Mouse, Primate, Rat
• Isotype: Mouse IgG2a
• Immunogen: recombinant human cyclin D1 (amino acids 1-295)
• Concentration: 1 mg/ml
• Dilution range: Immunohistochemistry: Recommended dilution: 5-10 μg/ml. Flow cytometry: Recommended dilution: 3-12 µg/ml. Intracellular staining.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: Cyclin D1
• Entrez: 595
• UniProt ID: P24385
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-CYC-D1 antibody, anti-CCND1 antibody, anti-Bc
• Note: For research use only
• Application notes: Immunohistochemistry: Recommended dilution: 5-10 μg/ml. Flow cytometry: Recommended dilution: 3-12 µg/ml. Intracellular staining.
• Expiration Date: 12 months from date of receipt.

Separation of HeLa cells stained using anti-Cyclin D1 (DCS-6) purified antibody (concentration in sample 9 µg/ml, GAM APC, red-filled) from HeLa cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).