CCND1 Antibody

• Catalog Number: orb1252470
• Category: Antibodies
• Description: CCND1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CCND1/809
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG2a, kappa
• Immunogen: Recombinant human protein was used as the immunogen for the Cyclin D1 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the Cyclin D1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CCND1
• UniProt ID: P24385
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: G1/S-specific cyclin-D1, B-cell lymphoma 1 protein
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlWestern blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the Cyclin D1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Formalin-fixed, paraffin-embedded human mantle cell lymphoma stained with Cyclin D1 antibody (CCND1/809).

Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Cyclin D1 antibody (CCND1/809).

Flow cytometry of human HeLa cells. Black: cells alone; Green: isotype control; Red:PE-labeled Cyclin D1 antibody (CCND1/809)

Flow cytometry of human Jurkat cells. Black: cells alone; Grey: isotype control; Green: AF488-labeled Cyclin D1 antibody (CCND1/809).

SDS-PAGE Analysis of Purified, BSA-Free Cyclin D1 Antibody (clone CCND1/809). Confirmation of Integrity and Purity of the Antibody.