Cyclin D1 antibody

• Catalog Number: orb10496
• Category: Antibodies
• Description: Cyclin D1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IHC-P, WB
• Predicted Reactivity: Canine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Cyclin D1 (61-110/295 aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, ICC=1:100, Flow-Cyt=1μg/Test, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 32 kDa
• Target: Cyclin D1
• UniProt ID: P25322
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: CyclinD1; Cyclin-D1; B cell ccl/lymphoma 1; B cell
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Mejía-Rodríguez, Rosalinda et al. Combined treatments with AZD5363, AZD8542, curcumin or resveratrol induce death of human glioblastoma cells by suppressing the PI3K/AKT and SHH signaling pathways Biochem Biophys Rep, 33, 101430 (2023)

PubMed: 36714540

Applications

WB

Reactivity

Human

Domínguez-Calderón, Alaide et al. ZO-2 silencing induces renal hypertrophy through a cell cycle mechanism and the activation of YAP and the mTOR pathway Mol Biol Cell, 27, 1581-1595 (2016)

PubMed: 27009203

Applications

WB

Reactivity

Canine

Zhang, Peng et al. Knockdown of Rho-associated protein kinase 1 suppresses proliferation and invasion of glioma cells Tumour Biol, 36, 421-428 (2015)

PubMed: 25266804

Applications

WB

Reactivity

Human

IF analysis of MCF7 cell lysate using Cyclin D1 antibody

IHC-P image of human placenta tissue using Cyclin D1 antibody

IHC-P image of human endometrium carcinoma tissue using Cyclin D1 antibody

IHC-P image of Human cervical cancer tissue using Cyclin D1 antibody

IHC-P image of Rat testis tissue using Cyclin D1 antibody

IHC-P image of Rat esophageal tissue using Cyclin D1 antibody

IHC-P image of rat liver tissue using Cyclin D1 antibody

IHC-P image of rat liver tissue using Cyclin D1 antibody

IHC-P image of rat kidney tissue using Cyclin D1 antibody

WB analysis of human A549 cell Lysate at 30 ug using Cyclin D1 antibody

WB analysis of Lane 1: Small intestine (Mouse) Lysate at 40 ug, Lane 2: U251 (Human) Cell Lysate at 30 ug, Lane 3: HT1080 (Human) Cell Lysate at 30 ug using Cyclin D1 antibody

WB analysis of A549 lysate at 40ug using Cyclin D1 antibody

WB analysis of human MCF-7 lysate using Cyclin D1 antibody

IF analysis of MCF7 cell lysate using Cyclin D1 antibody