CCND1 Antibody

• Catalog Number: orb1252826
• Category: Antibodies
• Description: CCND1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: DCS-6
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human, Monkey, Mouse, Rat
• Isotype: IgG2a, kappa
• Immunogen: Human recombinant full length protein was used as the immunogen for this Cyclin D1 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cyclin D1 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CCND1
• UniProt ID: P24385
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: G1/S-specific cyclin-D1, B-cell lymphoma 1 protein
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cyclin D1 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of human mantle cell lymphoma stained with Cyclin D1 antibody. Nuclear staining of tumor cells is seen.

Western blot testing of human and mouse samples with DCS-6 mAb.

FACS testing of Jurkat cells: Black = cells alone; Green = isotype control; Red = Cyclin D1 antibody PE conjugate.

FACS testing of MCF-7 cells: Black = cells alone; Green = isotype control; Red = Cyclin D1 antibody PE conjugate.

SDS-PAGE Analysis of Purified, BSA-Free Cyclin D1 Antibody (DCS-6). Confirmation of Integrity and Purity of the Antibody.