Cyclin D1 Antibody / CCND1

• Catalog Number: orb2637517
• Category: Antibodies
• Description: This antibody recognizes a protein of 36kDa, identified as Cyclin D1. It is a putative proto-oncogene overexpressed in a wide variety of human neoplasms and a key cell cycle regulator. This antibody neutralizes the activity of Cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression. Cyclin D1 antibody is useful in identifying mantle cell lymphomas, which stain positive, from CLL/SLL and follicular lymphomas, which stain negative. Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2a, kappa
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: Human recombinant full length protein was used as the immunogen for this Cyclin D1 antibody.
• Tested applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cyclin D1 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: DCS-6
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Cyclin D1 antibody is available for research use only.
• Note: For research use only
• Entrez: 595

IHC testing of human mantle cell lymphoma stained with Cyclin D1 antibody. Nuclear staining of tumor cells is seen.

Western blot testing of human and mouse samples with DCS-6 mAb. Predicted molecular weight: 32-36 kDa.

FACS testing of Jurkat cells: Black = cells alone; Green = isotype control; Red = Cyclin D1 antibody PE conjugate (V2006PE)

FACS testing of MCF-7 cells: Black = cells alone; Green = isotype control; Red = Cyclin D1 antibody PE conjugate (V2006PE)

SDS-PAGE Analysis of Purified, BSA-Free Cyclin D1 Antibody (DCS-6). Confirmation of Integrity and Purity of the Antibody.