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Catalog Number | orb1267138 |
---|---|
Category | Antibodies |
Description | CRTAP Antibody |
Target | CRTAP |
Clonality | Polyclonal |
Isotype | Rabbit Ig |
Conjugation | Unconjugated |
Reactivity | Human |
Form/Appearance | Liquid |
Concentration | batch dependent |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Purification | This antibody is purified through a protein A column, followed by peptide affinity purification. |
Immunogen | This CRTAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-59 amino acids from the N-terminal region of human CRTAP. |
UniProt ID | O75718 |
MW | 47 kDa |
Tested applications | IF, WB |
Application notes | For IF starting dilution is: 1:25For WB starting dilution is: 1:1000 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Cartilage-associated protein, CRTAP, CASP |
Note | For research use only |
NCBI | O75718 |
Fluorescent image of Hela cells stained with CRTAP Antibody (N-term). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Western blot analysis of lysates from HT-1080, ZR-75-1 cell line (from left to right), using CRTAP Antibody at 1:1000 at each lane.
Western blot analysis in Hela cell line lysates (35 ug/lane).
ELISA, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |