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Catalog Number | orb1254959 |
---|---|
Category | Antibodies |
Description | CREB1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human CREB1 (NP_604391.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 43kDa |
Target | CREB1 |
UniProt ID | P16220 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | CREB1, CREB, MGC9284 Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of A-431 cells, using CREB1 antibody (orb1254959) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using CREB1 antibody (orb1254959) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using CREB1 antibody (orb1254959) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CREB1 antibody (orb1254959) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CREB1 antibody (orb1254959) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using CREB1 antibody (orb1254959) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
IH, IP, WB | |
Human, Mouse, Porcine, Rat, Zebrafish | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Porcine, Rat, Sheep, Zebrafish | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IHC-P, WB | |
Bovine, Canine, Gallus, Porcine, Sheep |
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