CREB (Phospho-S133) Rabbit Polyclonal Antibody

SKU: orb213775

Description

The CREB (Phospho-S133) Antibody is suitable for IHC, IP, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S133 of human CREB protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics, Infectious Diseases

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB: 1-500-1-1000, IHC-P: 1-100-1-200, IP: 1-10-1-100
• Reactivity: Bovine, Gallus, Human, Mouse, Porcine, Rat, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S133 of human CREB protein. The exact sequence is proprietary.
• Target: CREB1
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1

Western blot analysis of CREB (Phospho-S133) expression in C6 (A), NIH3T3 (B), HEK293T (C), H1792 (D) whole cell lysates. (Predicted band size: 35 kD; Observed band size: 43 kD)

Immunohistochemical analysis of CREB (Phospho-S133) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Direct ELISA antibody dose-response curve using Anti-CREB (Phospho-S133) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.

Quick Database Links

Gene Symbol

CREB1

UniProt

Q01147, P16220, P15337

NCBI Gene ID

12912, 81646, 1385

Wójcik-Pszczoła, Katarzyna et al. A Novel, Pan-PDE Inhibitor Exerts Anti-Fibrotic Effects in Human Lung Fibroblasts via Inhibition of TGF-β Signaling and Activation of cAMP/PKA Signaling Int J Mol Sci, 21, (2020)

PubMed: 32503342

Wójcik-Pszczoła, Katarzyna et al. Pan-Phosphodiesterase Inhibitors Attenuate TGF-β-Induced Pro-Fibrotic Phenotype in Alveolar Epithelial Type II Cells by Downregulating Smad-2 Phosphorylation Pharmaceuticals (Basel), 15, 423 (2022)

PubMed: 35455420

Lewosiński, E. et al. [Nasal glioma] Otolaryngol Pol, 35, 289-292 (1981)

PubMed: 7312375