Clusterin Antibody / Apolipoprotein J / APO-J

• Catalog Number: orb1716281
• Category: Antibodies
• Description: Clusterin, also designated complement lysis inhibitor (CLI), apolipoprotein J (APOJ), sulfated glycoprotein 2 (SGP2), SP40 and testosterone-repressed prostate message 2 (TRPM2), is a secretory, heterodimeric glycoprotein that influences immune regulation, cell adhesion, transformation, lipid transportation, tissue remodeling, membrane recycling and cell-cell interactions. Clusterin is synthesized as a 449 amino acid polypeptide that is post-translationally cleaved at an internal bond between Arg 227 and Ser 228. Two subunits, -Amyloid structure and neuritic toxicity in vivo and may influence Alzheimer s pathogenesis.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A portion of amino acids 150-300 from the human CLU protein was used as the immunogen for the Clusterin antibody.
• UniProt ID: P10909
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the Clusterin antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: CLU/4724
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Clusterin antibody is available for research use only.
• Note: For research use only

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using Clusterin antibody (clone CLU/4724). These results demonstrate the foremost specificity of the CLU/4724 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

IHC staining of FFPE human adrenal gland tissue with Clusterin antibody (clone CLU/4724). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Clusterin antibody (clone CLU/4724) as confirmation of integrity and purity.