CFL1 Antibody

• Catalog Number: orb1264519
• Category: Antibodies
• Description: CFL1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Gallus, Monkey, Porcine, Rat, Xenopus
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This CFL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-98 amino acids from the Central region of human CFL1.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 19 kDa
• Target: CFL1
• UniProt ID: P23528
• NCBI: P23528
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Cofilin-1, 18 kDa phosphoprotein, p18, Cofilin, no
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of CFL1 Antibody in HL-60 cell line lysates (35 ug/lane).

Western blot analysis of CFL1 Antibody in mouse NIH-3T3 cell line lysates (35 ug/lane)

Western blot analysis of CFL1 using rabbit polyclonal CFL1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CFL1 gene.

Flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.