Cofilin/CFL1 Antibody

• Catalog Number: orb182365
• Category: Antibodies
• Description: Cofilin/CFL1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, IHC-Fr, WB
• Predicted Reactivity: Bovine, Equine
• Reactivity: Human, Monkey, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Cofilin recombinant protein (Position: A2-L166). Human Cofilin shares 99% amino acid (aa) sequences identity with both mouse and rat Cofilin.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat, Monkey Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human ELISA , 0.1-0.5μg/ml, Human, -
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 18502 MW
• UniProt ID: P23528
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Cofilin-1;18 kDa phosphoprotein;p18;Cofilin, non-m
• Note: For research use only
• Application notes: WB: The detection limit for Cofilin is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U20S cells using anti-Cofilin antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Cofilin using anti-Cofilin antibody.Lane 1:human HeLa cell;2:monkey COS-7 cell;3:human HEK293 cell;4:human U-87MG cell;5:human HepG2 cell;6:human PC-3 cell;7:human THP-1 cell;8:human A549 cell.

WB analysis of Cofilin using anti-Cofilin antibody.Lane 1:rat heart tissue;2:rat spleen tissue;3:rat lung tissue;4:rat C6 cell;5:mouse spleen tissue;6:mouse NIH/3T3 cell;7:mouse HEPA1-6 cell.

IF analysis of Cofilin using anti-Cofilin antibody. Cofilin was detected in immunocytochemical section of U20S cell.

IHC analysis of Cofilin using anti-Cofilin antibody. Cofilin was detected in paraffin-embedded section of mouse lung tissues.

IHC analysis of Cofilin using anti-Cofilin antibody. Cofilin was detected in paraffin-embedded section of rat lung tissues.

IHC analysis of Cofilin using anti-Cofilin antibody. Cofilin was detected in paraffin-embedded section of human intestinal cancer tissues.

IHC analysis of Cofilin using anti-Cofilin antibody. Cofilin was detected in paraffin-embedded section of human mammary cancer tissues.

IHC analysis of Cofilin using anti-Cofilin antibody. Cofilin was detected in frozen section of human placenta tissues.