Cofilin/CFL1 Antibody

• Catalog Number: orb315128
• Category: Antibodies
• Description: Cofilin/CFL1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC, WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Monkey, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Cofilin (122-152aa DAIKKKLTGIKHELQANCYEEVKDRCTLAEK), identical to the related mouse and rat sequences.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Monkey, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 18502 MW
• UniProt ID: P23528
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Cofilin-1;18 kDa phosphoprotein;p18;Cofilin, non-m
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of Cofilin using anti-Cofilin antibody.Lane 1:human HeLa cell;2:human 293T cell;3:human U-87 MG cell;4:monkey COS-7 cell;5:rat brain tissue;6:rat C6 cell;7:mouse brain tissue.

IF analysis of Cofilin using anti-Cofilin antibody. Cofilin was detected in an immunocytochemical section of MCF-7 cells.

IHC analysis of Cofilin using anti-Cofilin antibody. Cofilin was detected in a paraffin-embedded section of human intestinal cancer tissue.

IHC analysis of Cofilin using anti-Cofilin antibody. Cofilin was detected in a paraffin-embedded section of mouse intestine tissue.

IHC analysis of Cofilin using anti-Cofilin antibody. Cofilin was detected in a paraffin-embedded section of rat intestine tissue.