CD80 Rabbit Polyclonal Antibody

• Catalog Number: orb10345
• Category: Antibodies
• Description: CD80 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD80 (111-265/288aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=2µg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 60-65 kDa
• Target: CD80
• UniProt ID: P33681
• RRID: AB_10750637
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Activation B7-1 antigen; B lymphocyte activation a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: Raji. Primary Antibody (green line): Rabbit Anti-CD80 antibody (orb10345), Dilution: 2 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: U937 (blue). Primary Antibody: Rabbit Anti-CD80 antibody (orb10345), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (orb10345, 1 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

Sample: HL-60 (Human) Cell Lysate at 40 ug, Primary: Anti-CD80 (orb10345) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 32 kD, Observed band size: 57 kD.

Sample: Lane 1: Raw264.7 (Mouse) Cell Lysate at 30 ug, Lane 2: SP2/0 (Mouse) Cell Lysate at 30 ug, Lane 3: Thymus (Rat) Lysate at 40 ug, Lane 4: Raji (Human) Cell Lysate at 30 ug, Lane 5: K562 (Human) Cell Lysate at 30 ug, Primary: Anti-CD80 (orb10345) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60-65 kD, Observed band size: 60 kD.