CD80 Rabbit Polyclonal Antibody

• Catalog Number: orb1675375
• Category: Antibodies
• Description: CD80 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant mouse CD80 protein (38-246/306aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 32 kDa
• Target: CD80
• UniProt ID: Q00609
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Activation B7-1 antigen; B lymphocyte activation a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: Mouse Lung tissue lysates, Lane 2: Human HL-60 cell lysates, Primary: Anti-CD80 (orb1675375) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 32 kDa, Observed band size: 68 kDa.

Measured by its binding ability in a indirect ELISA. Immobilized Mouse CD80 protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Mouse CD80 Antibody, the EC50 is 36.63 ng/ml.

Measured by its binding ability in a indirect ELISA. Immobilized Mouse CD80 protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Mouse CD80 Antibody, the EC50 is 31.10 ng/ml.