CD80 Rabbit Polyclonal Antibody

• Catalog Number: orb5805
• Category: Antibodies
• Description: CD80 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from rat CD80 (251-321/321aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=2µg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 60-65 kDa
• Target: CD80
• RRID: AB_10939108
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Activation B7-1 antigen; B lymphocyte activation a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

SP Dai, CC Yang, Y Chin, WH Sun TDAG8-mediated distinct signaling pathways modulate the early and late phases of neuropathic pain iScience, (2024)

PubMed:

Applications

IHC

Reactivity

Mouse

Sample: Lane 1: Raw264.7 (Mouse) Cell Lysate at 30 ug, Lane 2: SP2/0 (Mouse) Cell Lysate at 30 ug, Lane 3: Thymus (Rat) Lysate at 40 ug, Lane 4: Raji (Human) Cell Lysate at 30 ug, Lane 5: K562 (Human) Cell Lysate at 30 ug, Primary: Anti-CD80 (orb5805) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60-65 kD, Observed band size: 60 kD.

Sample: Lane 1: Raw264.7 (Mouse) Cell Lysate at 30 ug, Lane 2: SP2/0 (Mouse) Cell Lysate at 30 ug, Lane 3: Thymus (Rat) Lysate at 40 ug, Lane 4: Raji (Human) Cell Lysate at 30 ug, Lane 5: K562 (Human) Cell Lysate at 30 ug, Primary: Anti-CD80 at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60-65 kD, Observed band size: 60 kD.