CD8 antibody

• Catalog Number: orb222736
• Category: Antibodies
• Description: Rat monoclonal antibody to CD8
• Clonality: Monoclonal
• Clone Number: 53-6.7
• Tested applications: FA, FC, ICC, IHC-Fr, IHC-P, IP
• Reactivity: Mouse
• Isotype: Rat IgG2a kappa
• Immunogen: Murine spleen cells
• Concentration: 1 mg/ml
• Dilution range: Functional application: Isolation and depletion of CD8+ cells, blocking of cytotoxicity, inhibition of CD8+ T cell proliferation. Flow cytometry: Recommended dilution: 1.5 μg/ml. Immunohistochemistry (frozen sections): Recommended dilution: 1:1000; formaldehyde fixation is not recommended, acetone fixation is preferred.
• Purity: Purified by protein-G affinity chromatography.
• Conjugation: Unconjugated
• Target: CD8a
• Entrez: 12525
• UniProt ID: P01731
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Alternative names: anti CD 8 antibody, anti CD 8A antibody, anti CD 8
• Note: For research use only
• Application notes: Low Endotoxin, Azide free format. Product does not contain preservatives and is intended primarily for functional applications. Preservative containing buffers are available for IHC applications
• Expiration Date: 12 months from date of receipt.

Separation of murine CD8a positive splenocytes (red-filled) from myeloid cells (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD8a (53-6.7) purified antibody (low endotoxin, concentration in sample 3 μg/ml) DAR PE.

Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD8a (53-6.7) purified antibody (low endotoxin, concentration in sample 3 μg/ml) DAR PE.