CD45RO (T-Cell Marker) Antibody

• Catalog Number: orb2318318
• Category: Antibodies
• Description: CD45RO (T-Cell Marker) Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: UCHL-1 + T200/797
• Tested applications: IHC, WB
• Reactivity: Human
• Isotype: IgG2a
• Immunogen: Cultured human T-cells from an IL-2-dependent T-cell line (CA1) (UCHL-1); Human T-lymphocytes (T200/797)
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 180-185kDa
• Target: PTPRC
• Entrez: 5788
• UniProt ID: P08575
• Expression System: CHO cells
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: T200, GP180, LCA, Leukocyte Common Antigen, LY5, R
• Note: For research use only
• Application notes: Positive Control: CCRF-CEM, Jurkat or MOLT-4 cells. Human Spleen, Paracortex in a tonsil or a reactive lymph node.
• Expiration Date: 12 months from date of receipt.