CD45 antibody

• Catalog Number: orb44271
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD45
• Clonality: Monoclonal
• Clone Number: MEM-28
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Human thymocytes and T lymphocytes.
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: APC
• Target: CD45
• Entrez: 5788
• UniProt ID: P08575
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti CD 45 antibody, anti CD45 antibody, anti CD45
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Separation of human CD45 positive lymphocytes (red-filled) from human CD45 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD45 (MEM-28) APC (10 µl reagent / 100 µl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD45 (MEM-28) APC (10 µl reagent / 100 µl of peripheral whole blood).