CD45 Antibody

• Catalog Number: orb1519973
• Category: Antibodies
• Description: CD45 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: FC, ICC, IHC, IP, WB
• Reactivity: Human
• Immunogen: between 1250 to 1306 (C-term)
• Concentration: 50 µg/ml
• Dilution range: WB - 1:1000; IP - 20 µl/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: CD45
• UniProt ID: P08575
• NCBI: NP_002829.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: L-CA; protein tyrosine phosphatase, receptor type,
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human CD45 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, HEK293T, MOLT-4, HeLa, and SR cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (orb1519973) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

Detection of human CD45 by immunohistochemistry. Sample: FFPE section of appendix. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (orb1519973). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human CD45 by immunohistochemistry. Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (orb1519973). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human CD45 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (orb1519973) used for IP at 20 µl/mg lysate. CD45 was also immunoprecipitated by a previous lot of this antibody (orb1519973). For blotting immunoprecipitated CD45, orb1519973 was used at 1:1000. Chemiluminescence with an exposure time of 1 second.

Detection of human CD45 by immunocytochemistry. Sample: FFPE section of Jurkat cells. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (orb1519973). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human CD45 by immunocytochemistry. Sample: FFPE section of Daudi cells. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (orb1519973). Secondary: HRP-conjugated goat anti-rabbit IgG.