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Catalog Number | orb1294366 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to CD45 antibody (Intracellular domain) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IF, IHC, WB |
Reactivity | Human |
Isotype | Rabbit-IgG |
Immunogen | Synthetic peptide / Intracellular domain corresponding to Human CD45 |
Dilution range | Western Blot 1:500-1:1000 Immunofluorescence 1:200-1:500 Immunohistochemistry (Paraffin) 1: 200-1:400 Flow Cytometry 1:50-1:100 |
Conjugation | Unconjugated |
Target | CD45 (Intracellular) |
Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of paraffin embedded Human Gastric cancer tissue labeling CD45 with orb1294366 at 1:300.
Immunohistochemical analysis of paraffin embedded Human Ovary cancer tissue labeling CD45 with orb1294366 at 1:300.
Immunohistochemical analysis of paraffin embedded Human Tonsil cancer tissue labeling CD45 with orb1294366 at 1:300.
Immunohistochemical analysis of paraffin embedded Human Squamous cell carcinoma labeling CD45 with orb1294366 at 1:300.
Immunohistochemical analysis of paraffin embedded Human Lymph node carcinoma labeling CD45 with orb1294366 at 1:300.
Immunohistochemical analysis of paraffin embedded Human Lung cancer tissue labeling CD45 with orb1294366 at 1:300.
Immunohistochemical analysis of paraffin embedded Human Liver cancer tissue labeling CD45 with orb1294366 at 1:300.
Immunohistochemical analysis of paraffin embedded Human Breast cancer tissue labeling CD45 with orb1294366 at 1:300.
Immunofluorescence:cells were stained with anti-CD45 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunofluorescence:cells were stained with anti-CD45 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human Colorectal cancer tissue labeling CD45 with orb1294366 at 1:300.
Cell : THP-1
Western blot analysis using Anti-CD45 antibody at 1:1000 dilution, lysates/proteins at 50 μg per lane.
FACS, IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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