PTPRC Antibody

• Catalog Number: orb1252710
• Category: Antibodies
• Description: PTPRC Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 158-4D3
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG2a, kappa
• Immunogen: Stimulated human leukocytes were used as the immunogen for this CD45RA antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD45RA antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PTPRC
• UniProt ID: P08575
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Receptor-type tyrosine-protein phosphatase C, Leuk
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD45RA antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human tonsil with CD45RA antibody (clone 158-4D3). HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

IHC staining of FFPE human spleen with CD45RA antibody (clone 158-4D3). HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

FACS surface staining of human PBMCs using CD45RA antibody (clone 158-4D3, red), and isotype control (green).

Flow cytometry testing of human Jurkat cells with CD45RA antibody (clone 158-4D3); Red = isotype control, Blue = CD45RA antibody.

Western blot testing of human spleen lysate with CD45RA antibody (clone 158-4D3). Expected molecular weight: 147-220 kDa depending on glycosylation level.

SDS-PAGE analysis of purified, BSA-free CD45RA antibody (clone 158-4D3) as confirmation of integrity and purity.