CD45 antibody

• Catalog Number: orb1474239
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD45
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse
• Immunogen: Purified recombinant fragment of human PTPRC (AA: 928-989) expressed in E. Coli.
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100)
• Form/Appearance: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Conjugation: Unconjugated
• Target: PTPRC
• Entrez: 5788
• UniProt ID: P08575, P06800
• Source: Mouse
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Alternative names: CD45; Receptor-type tyrosine-protein phosphatase C
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CD45 expression in Ramos (A), Raw264.7 (B) whole cell lysates. (Predicted band size: 147 kD; Observed band size: 180-240kD)

Immunohistochemical analysis of CD45 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.34). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.