CD45 Antibody (Leukocyte marker)

• Catalog Number: orb2640041
• Category: Antibodies
• Description: CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PTPRC/1461
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: Human B lymphocytes were used as the immunogen for the CD45 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD45 antibody is available for research use only.
• UniProt ID: P08575
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Due to variation in protocol and secondary antibody used, the CD45 antibody may need to be titered for optimal performance.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of PFA-fixed human Jurkat cells with CD45 antibody (clone PTPRC/1461, green) and Reddot nuclear stain (red).

IHC staining of FFPE human tonsil with CD45 antibody (clone PTPRC/1461). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Flow cytometry testing of PFA-fixed human Jurkat cells with CD45 antibody (clone PTPRC/1461); Red = isotype control, Blue = CD45 antibody.

SDS-PAGE Analysis of Purified, BSA-Free CD45 Antibody (clone PTPRC/1461). Confirmation of Integrity and Purity of the Antibody.